Objective  To establish the magnetic activated cell sorting system (MACS)for isolation and incubation of human ovarian carcinoma-derived microvascular endothelial cells (ODMECs) and to observe their ability to form tubule-like structure (TLS) in vitro.   Methods  MACS was used to purify ODMECs with antiCD31 antibody and ODMECs were identified according to their morphological，biochemical and phenotypic characteristics. The ability of ODMECs to intake acetylated-low-density lipoprotein (DIL-acLDL) and Ulex europeaus agglutinin-1 (UEA-Ⅰ) was assayed to observe the formation of TLS in vitro.   Results  Flow cytometry showed that the purity of ODMECs was up to 99.6%. The ODMECs were characterized by cobble stones

contact inhibition

expressed CD31，CD105 and FⅧ-Rag

and exhibited the ability to bind to UEA-1

intake DIL-acLDL and form TLS during incubation.   Conclusion  The MACS we established can be used to isolate and incubate human ODMECs and study the formation characteristics of TLS.