Objective    To study the role of   Cysteine-rich 61 (Cyr61/CNN1) in repair of combined injury by observing whether exogenous CCN1 promotes the proliferation and migration of radiation-injured L929 cells.     Methods    A radiation model of L929 cells was induced by γ ray at a dose of 4 Gy. The irradiated L929 cells were cultured in a medium containing 2 ml adenovirus plasmids of CCN1 or RFP at 37 ℃ in an atmosphere containing 5%CO

2

which served as a CCN1 group and a RFP group

respectively. Irradiated L929 cells cultured in a blank

control medium served as a blank control group. Effects of CCN1 on proliferation and migration of radiationinjured L929 cells were detected by MTT assay

plate colony formation assay

cell cycle analysis

and scratch test of wound healing.     Results    The proliferation and colony formation rates of L929 cells cultured in a medium containing CCN1 were significantly higher than in RFP and control groups [colony formation rate: (34.4±3.6)% vs (24.5±2.9)% and (29.5±3.5)%

P0.05

]

. Five hours after culture

the amount of L929 cells in the S phase was larger in CCN1 group than in RFP and control groups (37.3%±2.3% vs 252%±1.9% and 26.9%±1.3%

P0.01). Scratch test of wound healing showed that the width/thickness ratio of migrated L929 cells in healed wounds was significantly higher in CCN1 group than in RFP and control groups (P0.01).     Conclusion    Exogenous CCN1 promotes the proliferation and migration of radiation-injured L929 cells

suggesting that CCN1 is one of the stimulating factors for the repair of combined injury.