Objective   To prepare the bioactive monoclonal antibody (McAb) against the fusion protein of GST-human I-alanine aminotransferase (ALT1).    Methods   With the fusion protein GST-ALT1 as immunogen

the anti-ALT1 McAb was produced by using the hybridoma technique

of which the antigen binding characteristics was identified by indirect enzyme-linked immunosorbent assay (ELISA)

Dot-ELISA and Western blot assay．   Results   One cell strain of hybridoma was obtained and named as 8A9 which secreted the anti-ALT1 McAb. The class and subtype identification demonstrated that this cell strain was IgG2．The titers of ascites fluid which hybridoma induced was 10×10

5

. Dot-ELISA and Western blotting demonstrated that this antibody recognized speci

fically the immunogen and ALT1 which existed in clinical serum specimen.    Conclusion   McAb against ALT1 is prepared successfully and provides a useful tool for researching on ALT1’s property

function

bionomics and found a basis for establishment of specific immunoassays of human ALT1.