Objective  To observe the localization of transplanted human umbilical cord-matrix stem cells (UC-MSCs) in mouse premature ovarian failure (POF) model.  Methods  POF animal model was established by cyclophosphamide (CPA) and busulfan (BUS) chemotherapy. After UC-MSCs were isolated and proliferated from UC

recombinant Ad-enovirus-green fluorescent protein (Ad-GFP) was transfected in them via Ad-enovirus. The GFP-transfected UC-MSCs (2×10

6

to 3×10

6

) were transplanted into POF mice through caudal vein

and tissues from POF mice were obtained for frozen section at 1

10 and 20 d after

transplantation. GFP-positive cells in the ovary

brain

liver

kidney and pancreas were determined by laser confocal microscopy.  Results  Transfection efficiency of Ad-GFP by Ad-enovirus was about 95%. After transplantation

GFP-positive cells were found in the harmed ovaries and more GFP-positive cells were found at 10 and 20 d after transplantation than those at 1 d after transplantation. However

no GFP-positive cells were found in the bone marrow

brain

liver

kidney and pancreas. Conclusion  Human UC-MSCs can home and survive in the ovaries of POF mouse model. It suggests that UC-MSCs maybe have the potential for POF therapy.