Objective   To explore the effect of curcumine on the proliferation and heparanase expression in blood vessel endothelium cells isolated from ovarian cancer tissues.    Methods   Blood vessel endothelium cells were obtained from pathologically identified ovarian cancer tissues by primary culture. The control group was the normal blood vessel endothelium cells. The experimental groups was treated with curcumine at 2

10

50  and 250 μg/ml for 24

48 h and 72 h respectively. The cytostasis rate and cell cycle changes of the primarily cultured cells were detected by MTT assay and flow cytometry respectively. The transcriptional and translation levels of heparanase were detected by RT-PCR and immunofluorescent staining. The MDA content and LDH activity in the supernatant were detected too.    Results   In the curcumine groups

the cell proliferation was inhibited in a dose-dependent and time-dependent manner (P0.01). Cell proliferation index was decreased significantly in the same manner (P0.01). According to the immunofluorescence assay and RT-PCR

the heparanase expression in the curcumine groups was decreased significantly (P0.01). The MDA content and LDH activity in the cell culture supernatant was significantly increased (P0.01).    Conclusion   Curcumine at the dose of 2 to 250 μg/ml significant inhibits the proliferation and heparanase expression in the blood vessel endothelium cells from ovarian cancer in a dose-dependent and time-dependent manner.