Objective   To explore the key technologies for the construction of engineered osteochondral tissue in vitro with tissue engineering principles.    Methods   With the aid of tissue laminated construction strategy

rabbit osteoblasts and hydroxyapatite ceramic (HAC) scaffold were used to construct tissue engineered bone in vitro with proteic-gel. Rabbit chondrocytes and PLLA/CPPf scaffold were employed to construct tissue engineered cartilage in vitro with proteic-gel. After cultured for 48 h

the engineered osteochondral tissue was prepared by press gomphosis between the 2 uneven interfaces and then pasted with albumin gel. Then the construct-ed was transplanted into nude mouse subcutaneously. Control group was made with the same composite scaffold but without sells. In 12 weeks after operation

the transplanted engineered osteochondral tissue was taken out for pathological analysis.    Results   The tissue engineered bone and cartilage were primary constructed in vitro with proteic-gel. The engineered osteochondral tissue was primary constructed. After transplantion into subcutaneous tissue for 12 weeks

all samples in the experiment group displayed bone and cartilage formation in the correspond ing area

but the calcified cartilage zone was not found on the interface of the bone and cartilage. However

the cartilage formation was not observed in the control group.    Conclusion    The engineered osteochondral tissue is primary constructed in vitro with the tissue laminated construction strategy and proteic-gel technology.