Objective   To establish a rapid

specific and simple method for the quantitive determination of berberine hydrochloride.     Methods   Proton nuclear magnetic resonance spectroscopy (

1

H-NMR) spectra were obtained in CD

3

OD with a Varian Mercury Plus 400 MHz spectrometer at 25 ℃. For each sample

64 scans were recorded with the following parameters: observing frequency (400.123 MHz)

spectra width (6 410.3 Hz)

90° pulses width (6. 450 μs)

acquisition time (4 s) and delay time (18 s).     Results   The analysis of

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H-NMR spectrum of berberine hydrochloride mixed with 2

4-dinitrotoluene (2

4-DNT) as internal standard

in CD

3

OD revealed that the integration of signals at δ6.13 ppm of berberine hydrochloride and δ8.45 ppm of 2

4-DNT could be used for the quantitation. The precision RSD was 1.785% (n=6). The results of the contents were 88.847 7%、88.380 3% and 88.658 7%.     Conclusion  

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H NMR can be used for the quantitative determination and quality control of berberine hydrochloride.