Objective    To investigate whether ultrasoundmediated microbubble destruction can effectively deliver pEGFP-N

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plasmid into human gingival fibroblasts (HGFs).     Methods    The primary cultured HGFs were divided into 4 groups

that is

plasmid

microbubble+plasmid

ultrasound+plasmid

and ultrasound+microbubble+plasmid groups. pEGFP-N

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plasmid was used to transfect to HGFs as a gene marker with ultrasoundmediated microbubble destruction. The last group was further divided into subgroups to optimize the transfection conditions. After 48 h

phase-contrast fluorescent microscopy was empl

oyed to evaluate the expression of green fluorescent protein (GFP). The cell vitality was measured by the MTT assay.     Results    The transfection efficiency of the ultrasound+microbubble+plasmid group was higher than other experiment groups. Optimal gene expression was found when ultrasound was radiated at 1.5 W/cm

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for 60 s

microbubble was at a concentration of 10% and plasmid was at a concentration of 6.67 μg/ml

and the transfection efficiency was highest under this condition.     Conclusion    Under specific conditions

ultrasound mediated microbubble destruction enhances the reporter gene transfection and expression in HGFs.