Objective   To construct a virtual reality modeling language (VRML) model of larynx and implement remote 3D anatomical virtual manipulation of larynx.    Methods   Partial datasets of larynx were generated based on the second CVH slices with manually segmentation. Surface models were established with Amira 4.1 software and exported as VRML files. Each model was defined with some node

such as name

appearance and event-router. Then individual models were combined as an integrated VRML model. Parameters transmission between webpage and VRML could be achieved with JavaScript function. As a result

the visible attribute of VRML models became controllable and the remote anatomical virtual simulation of larynx could be performed.    Results   VRML-based VR model of larynx was established. 3D representation and virtual anatomical operation were validated with VRML plug-in.    Conclusion   Anatomical VRML model can be created with 3D reconstruction techniques based on cross-sectional datasets and 3D interactive virtual tele-operations can be implemented within this digital model

which offer a new method for long distance anatomical education based on virtual reality.