Objective    To explore the inhibitory effects of RNAi targeting survivin to suppress the expression of survivin and to induce the apoptosis of human renal cancer cell line 786-O.     Methods    The target gene fragments were cloned into pcDNA

TM

6.2-GW/EmGFPmiR vectors

and then the recombinant eukaryotic expression vectors were transfected into 786-O cells with liposomes. The expression changes in survivin mRNA and protein were detected by RT-PCR and immunohistochemical assay

and the proliferation inhibitory rate (IR) of 786-O cells was detected by M’TT assay.     Results    The mRNA and protein expressions of survivin in the siRNA group were significantly inhibited by the eukaryotic express

ion vectors

and it also inhibited significantly the growth and proliferation of 786-O cells.     Conclusion    Silencing survivin gene by the RNAi technique can not only effectively decrease the expressions of survivin gene and protein

but also inhibit the cell proliferation in 786-O cells. This may provide the experimental evidence for the survivin gene as a target of gene therapy in renal cancer.