Objective    To explore the feasibility

efficiency and effect of wide-type p53 gene transfected into cervical cancer HeLa cells by ultrasound microbubble intensifier.     Methods    Based on the  optimum parameter of ultrasound wave irradiation (0.5 W/cm

2

30 s) selected in preexperiment

HeLa cells were divided into 5 groups: (A)simple plasmid group

(B)plasmid-ultrasound group

(C)plasmid-ultrasound-microbubble group

(D)plasmid-liposome gr

oup

(E)blank control group. At 24-48h after transfection

the transfection efficiency was detected by fluorescence microscope

the mRNA expression of p53 was detected by RT-PCR

the cell cycle was detected by flow cytometry

and the inhibition rate of cells growth was detected by MTT assay.     Results    Fluorescence microscopy showed that the expression of green fluorescent protein in group C was higher than that in group Band group D (P0.05)

and there were no green fluorescent cells in group A and group E. RT-PCR confirmed that the specific p53 electrophoresis strips were seen in group C and group D. The optical density of the strip from group C was higher than that from group D (P0.05). The cell cycles of transfected p53 cells arrested from G

1

to S phase and the growth of cells transfected with wtp53 gene were dramatically inhibited. The characteristic of cells in group C and D was quite different from that of group E (P0.05).     Conclusion    The transfection efficiency of foreign p53 gene into HeLa cells can be enhanced by optimum ultrasound intensity and microbubble dosage. Wtp53 gene can get the cell cycle arrested in G

1

phase and inhibit the growth of  HeLa cells.