LIANG Zi-wen,ZHOU Guang-ju,YANG Zong-cheng,CHEN Jian,CHEN Yu.Identification of EOLA1 gene promoter sequence[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(23):2309-2311.
LIANG Zi-wen,ZHOU Guang-ju,YANG Zong-cheng,CHEN Jian,CHEN Yu.Identification of EOLA1 gene promoter sequence[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(23):2309-2311.DOI:
Objective To identify the promoter sequence of endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1) gene and to elucidate the molecular mechanisms controlling EOLA1 expression. Methods A DNA fragment containing 1 723 bp 5′ upstream of the EOLA1 gene and the transcription start site was generated by polymerase chain reaction and then cloned into a luciferase reporter gene vector
pGL3-basic. The relative luciferase activities driven by this 5′-upstream fragment and a series of deletion mutants were measured in transiently transfected human ECV304 cells
respectively. At last
the 1 723 bp upstream of the EOLA1 gene was analyzed online with Cluster Buster. Results A fragment 785 bp upstream of the EOLA1 coding region was sufficient to promote transcription. Further deletion analysis of the 785 bp fragment indicated that a 68 bp element from -738 to -676 was important for EOLA1 transcription in ECV304 cells. The 1 723 bp sequence contains binding sites for Sp1 and Myf. Conclusion We map the EOLA1 promoter by deletion analysis and reveal that the proximal region (-738 to -676 bp)
which contains binding sites for Sp1 and Myf
is essential for human EOLA1 promoter activity in ECV304 cells.