Objective   To investigate the performance of the demineralized bone matrix decorated with poly-L-lysine (PLL-DBM) in order to develop a type of enrichment matrix with good enrichment effects of bone marrow stem cells for selective cell retention (SCR) technology.   Methods   Cancellous bones harvested from the goat proximal femurs were used to remove the bone cortexes and then prepared to DBM. PLL was used to decorate the surface and interspace of DBM. Electron scanning microscopy was used to observe the structure of PLL-DBM

while amine acid component of PLL-DBM were analyzed to observe the compound condition of PLL and DBM. Colony forming unit-fibroblast (CFU-F) was calculated to determine the enrichment effect of PLL-DBM on bone marrow stem cells. Osteogenic ability of PLL-DBM was observed in goat lumbar intertransverse graft bone model by X-ray and histological analysis.   Results   PLL-DBM had a natural mesh pore structure; and PLL formed spider web-like mesh structure in the interspaces. PLL was well bond with DBM. The enrichment ability of PLL-DBM for bone marrow stem cells was superior to that of DBM (P0.01); Osteogenic ability of PLL-DBM was same as that of autologous bones.   Conclusion   The structure of PLL-DBM is profit to adhere marrow cells

which have better enrichment effect on marrow cells

and is a good enrichment matrix.