Objective   To investigate the therapeutic effect of transplantation of limbal epithelial cells and decellularized porcine cornea construct in rabbits with corneal alkali burn.    Methods   Allogeneic rabbit limbal epithelial cells were cultured and identified. Porcine cornea scaffold was fabricated by decellularization.The LECs-DPC construct was cultured and transplanted to the alkali-burned corneal surface of rabbits (4month follow-up).    Results   Immunocytochemistry and RT-PCR analyses revealed the presence of ABCG2 positive limbal stem cells. A general view showed the corneas were clear and vessel-free postoperatively . HE and Masson Trichrome staining results indicated the epithelial stratification

fewer cellular infiltration

DPC’ integration into host corneal tissues

and more regular collagen configuration. Transmission electron microscopy exhibited formation of desmosomes and microvilli.    Conclusion   Allogeneic limbal epithelial cells transplantation using DPC as a carrier can restore corneal clarity after alkali burn in a rabbit model.