Objective    To observe the effects of RNAi-mediated focal adhesion kinase (FAK) gene silencing on laryngeal carcinoma Hep-2 cells.     Methods    Recombinant plasmids generating short hairpin RNA were constructed

including two interferential sequences FAK-pGenesil-CH1

FAK-pGenesil-CH2 and one negative control sequence FAK-pGenesil-HK. Hep-2 cells were transfected with recombinant plasmids. FAK mRNA and protein were

analyzed respectively by RT-PCR and Western blotting. Cell proliferation

cell cycle and invasion of Hep-2 cells were observed by MTT assay

flow cytometry and transwell invasion assay

respectively.     Results    Recombinant plasmids of FAK-pGenesil-CH1 and FAK-pGenesil-CH2 downregulated the expressions of FAK mRNA and protein (P0.05). The proliferation inhibition ratio in Hep-2 cells reached 58.34% and 52.78% 48 h after transfection of recombinant plasmids of FAK-pGenesil-CH1 and FAK-pGenesil-CH2

while that was 8.34% in the control (P0.05). More cells arrested in G

0

/G

1

phase in the transfected groups than controls. The number of FAK-pGenesil-CH1 transfected Hep-2 cells

and FAK-pGenesil-CH2 transfected Hep-2 cells through Matrigel membrane was (56.0±6.7) and (51.0±5.1)

respectively

significantly less than that in control groups (P0.05).     Conclusion    The constructed recombinant plasmids of FAK-pGenesil-CH1 and FAK-pGenesil-CH2 can suppress the expression of FAK mRNA and protein in Hep-2 cells

and inhibit the cloning and invasion of Hep-2 cells.