Caveolin-1 RNA干扰慢病毒载体的构建及其对肝癌细胞株SMMC7721侵袭的影响

张志波; 蔡磊; 郑树国; 熊燕; 董家鸿

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Caveolin-1 RNA干扰慢病毒载体的构建及其对肝癌细胞株SMMC7721侵袭的影响

论著 | 更新时间：2026-01-09

- Caveolin-1 RNA干扰慢病毒载体的构建及其对肝癌细胞株SMMC7721侵袭的影响
- Construction of recombinant lentivirol expression vector carrying human Caveolin-1 with RNA interference and its effect on invasiveness of SMMC7721 cells
- 陆军军医大学学报 2009年31卷第10期页码：931-934
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  中图分类号： R394-33;R730.23;R735.7
- 纸质出版：2009
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张志波,蔡磊,郑树国,熊燕,董家鸿.Caveolin-1 RNA干扰慢病毒载体的构建及其对肝癌细胞株SMMC7721侵袭的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(10):931-934.

　ZHANG Zhi-bo,CAI Lei,ZHENG Shu-guo,XIONG Yan,DONG Jia-hong.Construction of recombinant lentivirol expression vector carrying human Caveolin-1 with RNA interference and its effect on invasiveness of SMMC7721 cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(10):931-934.
张志波,蔡磊,郑树国,熊燕,董家鸿.Caveolin-1 RNA干扰慢病毒载体的构建及其对肝癌细胞株SMMC7721侵袭的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(10):931-934. DOI：

　ZHANG Zhi-bo,CAI Lei,ZHENG Shu-guo,XIONG Yan,DONG Jia-hong.Construction of recombinant lentivirol expression vector carrying human Caveolin-1 with RNA interference and its effect on invasiveness of SMMC7721 cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(10):931-934. DOI：

摘要

目的构建人Caveolin-1 RNA干扰慢病毒载体，探讨慢病毒介导的Caveolin-1 RNA干扰对人肝癌细胞株SMMC7721侵袭能力的影响。方法应用pGCSIL-GFP-Caveolin-1 RNA干扰慢病毒载体感染SMMC7721，检测其转导效率、mRNA和蛋白水平的敲除效率，并检测对SMMC7721侵袭能力的影响。结果 pGCSIL-GFP-Caveolin-1 RNA干扰慢病毒载体感染SMMC7721的转导效率为(96.0±1.2)%；Caveolin-1 mRNA水平的敲除率约90%；Western blot显示感染后Caveolin-1蛋白水平显著下降；感染后SMMC7721的侵袭能力显著下降。结论慢病毒载体构建的Caveolin-1 RNA干扰能有效敲除SMMC7721中Caveolin-1的表达，显著降低SMMC7721侵袭能力。

Abstract

Objective To construct a lentivial expression vector of human Caveolin-1 with RNA interference and study its effect on the invasiveness of human hepatoma cell line SMMC7721. Methods After the lentiviral expression vector of human Caveolin-1 with RNA interference (pGCSIL-GFP-Caveolin-1 RNAi) was transduced into SMMC7721 cells

the transduction efficiency

the Caveolin-1 mRNA level were determined by real-time PCR

the expression of Caveolin-1 protein was measured by Western blot analysis. Its significance for the invasiveness of SMMC7721 cells were detected with the aid of Transwell. Results The transduction efficiency of our constructed vector pGCSIL-GFP-Caveolin-1 RNAi to SMMC7721 cells was (96.0±1.2)%. The Caveolin-1 mRNA level was knocked down by 90%. The expression of Caveolin-1 protein was significantly declined. The invasiveness of SMMC7721 cells was significantly descented. Conclusion A recombinant lentivirol expression vector carrying human Caveolin-1 with RNA interference is successfully constructed

and it knocks down the expression of Caveolin-1 in SMMC7721 cells efficiently and decrease the invasiveness of the cells significantly.

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