Objective   To explore the possibility of piezoelectric microarray immunosensor for the detection of Agkistrodon acutus venom.     Methods   Microarray immunosensor with quartz crystal of 10 MHz AT-cut and 2×5 gold-coated electrodes was prepared. The thiol-treated venom antibody was immobilized by a self assembling device for the detection of the standard fluid for different concentrations of the venom.     Results   Experimental results showed that the optimal concentration of the antibody was 3.0  g/ml with the response time of 40 minutes. The piezoelectric immunosensor could well respond to homologous venoms. Within the range of 0.1~4.0  g/ml

the frequency shifts were linearly dependent on the venom concentration.     Conclusion   Piezoelectric microarray immunosensor for the detection of Agkistrodon acutus venom is of high specificity of response

high sensitivity

and simple operation without marking. The technique of piezoelectric microarray immunosensor is possible to test snakebite quickly

quantitatively

and instrumentally.