Objective   To investigate the antimicrobial properties of human amniotic homogenate supernatant (HAHS) in order to found a theoretical base for the use of human amniotic membrane in ophthalmological field.    Methods   Fresh human amniotic membranes were used to make HAHS and acellular amniotic membranes. Then

we observed their antimicrobial effects and antimicrobial spectrums

compared the antimicrobial capacity with 10 commonly used antibiotics in eyes

and investigated the effects of time

temperature and pH value on the antimicrobial capacity. Finally

transmission electron microscopy (TEM) was used to explore the possible targeting site of the antibiosis.    Results   Human amnion membrane contained antimicrobial components locating in its epithelial cells. HAHS had a broad antimicrobial spectrum and was steady in nature. Its antimicrobial capacity was stronger than those of sulfasulfonamide

chloromycetin and cefuroxime sodium. TEM indicated that the antimicrobial effect were exerted through plasma membrane of microorganism.    Conclusion   HAHS can be an effective and convenient treatment for ocular surface infectious diseases. Traditional amnion transplantation should employ fresh human amniotic membranes containing complete epithelial lamina to reconstruct the ocular surface.