Objective    To study the toxicity

wound infection ability and antibiotic sensitivity of Eikenella corrodens isolated from maritime space of China.     Methods    Kunming mice were intraperitoneally injected with Eikenella corrodens suspension

and their general status was observed and leukocyte count and blood culture were preformed. On the fifth day after infection

mice were sacrificed and their organs were treated with formalin fixation

paraffin imbed

ding

HE staining and observed under light microscope. In toxicity experiment

the vulnerated limbs of Kunming mice were soaked in artificial sea water containing 2.0×10

6

CFU/ml Eikenella corrodens for 45 to 60 min

then they were raised in laminar flow cabinet

and their leukocyte count

blood culture and general status were surveyed at different time points after soaking

and their organs were tested too. The antibiotic susceptibility of Eikenella corrodens was tested by traditional K-B methods.     Results    After injected with Eikenella corrodens

the leukocytes of mouse were increased

and the liver and heart of 20% mice were Eikenella corrodens positive 12 h after infection. The diseased regions of lung

liver and kidney were more serious than other organs

and appeared hyperemia

hemorrhage and stasis because of dysaemia. Injured limb cultures of Kunming mice 6

12

24

48

72 h after soaking were all positive. In addition

the intestine cultures from 20% mice were positive 48 h after the injured limb was soaked. Loose arrangement

disorder

interruption of muscle fiber and inflammatory cell infiltration between striped muscles were also observed from injured limb.     Conclusion    Eikenella corrodens isolated from maritime space of China were virulent. Except for lower sensibility about 67% to Trimesulf

piperacillin

Meropenem and sulfanilamide

our isolated Eikenella corrodens displays good antibacterial activity to other antibiotics.