CXCR4/SDF-1对鼻咽癌细胞增殖与迁移能力的影响

邱际华; 刘求真; 陈丽; 焦锋; 姚开泰

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CXCR4/SDF-1对鼻咽癌细胞增殖与迁移能力的影响

论著 | 更新时间：2026-01-09

- CXCR4/SDF-1对鼻咽癌细胞增殖与迁移能力的影响
- Effect of CXCR4/SDF-1 on proliferation and migration of human nasopharyngeal carcinoma cell lines
- 陆军军医大学学报 2009年31卷第7期页码：598-600
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  中图分类号： R329.28;R73-37;R739.63
- 纸质出版：2009
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邱际华,刘求真,陈丽,焦锋,姚开泰.CXCR4/SDF-1对鼻咽癌细胞增殖与迁移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(07):598-600.

　QIU Ji-hua,LIU Qiu-zhen,CHEN Li,JIAO Feng,YAO Kai-tai.Effect of CXCR4/SDF-1 on proliferation and migration of human nasopharyngeal carcinoma cell lines[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(07):598-600.
邱际华,刘求真,陈丽,焦锋,姚开泰.CXCR4/SDF-1对鼻咽癌细胞增殖与迁移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(07):598-600. DOI：

　QIU Ji-hua,LIU Qiu-zhen,CHEN Li,JIAO Feng,YAO Kai-tai.Effect of CXCR4/SDF-1 on proliferation and migration of human nasopharyngeal carcinoma cell lines[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(07):598-600. DOI：

摘要

目的观察鼻咽癌不同恶性程度的细胞中趋化因子受体CXCR4 的表达

检测CXCR4/SDF- 1 反应轴对鼻咽癌细胞的增殖作用

SDF- 1对CXCR4阳性肿瘤细胞的趋化作用

探讨 CXCR4 /SDF-1生物学轴对人鼻咽癌细胞增殖与迁移能力的影响。方法以鼻咽癌成瘤高转移细胞株5-8F及成瘤不转移细胞株6-10B为研究对象，采用Western blot免疫印迹法检测鼻咽癌细胞株CXCR4蛋白的表达情况，SDF-1及CXCR4阻断剂AMD3100作用于两种细胞后

MTT法检测细胞的增殖能力

体外迁徙实验检测 CXCR4/SDF- 1反应轴对鼻咽癌细胞的趋化作用。结果 5-8F细胞株CXCR4蛋白的表达明显高于6-10B细胞株； SDF-1能明显增强5-8F细胞增殖与迁移能力， CXCR4阻断剂AMD3100作用后

5-8F细胞增殖与迁移能力明显降低；SDF-1对6-10B细胞的迁移及增殖能力无明显影响。结论 CXCR4 /SDF-1生物学轴与鼻咽癌细胞株的增殖与迁移有一定的关系，AMD3100可明显抑制鼻咽癌细胞的增殖与迁移能力。

Abstract

Objective To detect the expression of chemokine receptors 4 (CXCR4) in human nasopharyngeal carcinoma cell lines (NPC cells) and explore the role of CXCR4/stromal cell-derived factor-1 (SDF-1) axis in the proliferation and migration of tumor cell. Methods The expression of CXCR4 protein in NPC cell lines 5-8F (with high tumogenicity and metastasis) and 6-10B (having tumogenicity but no metastasis) was measured by Western blot analysis. After the addition of SDF-1 and CXCR4 blocker

AMD3100

the changes of cell growth were observed by MTT. The changes of cell migration were measured by Transwell. Results Western blotting showed that 5-8F cells expressed higher CXCR4 levels than 6-10B cells. The growth rate and the number of migration cells in 5-8F cells was increased after SDF-1 treatment

and significantly decreased after the addition of AMD3100. However

6-10B cells had no response to SDF-1. Conclusion CXCR4/SDF-1 axis may play an important role in NPC cells proliferation and migration

and CXCR4 blocker can efficiently suppress this effect.

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