腺苷A<sub>2A</sub>受体在小鼠大脑中动脉局灶缺血损伤中的作用及机制

田洪; 段炜; 桂莉; 郑健

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腺苷A_2A受体在小鼠大脑中动脉局灶缺血损伤中的作用及机制

论著 | 更新时间：2026-01-09

- 腺苷A_2A受体在小鼠大脑中动脉局灶缺血损伤中的作用及机制
- Role of adenosine A_2A receptor in ischemic brain injury induced by transient middle cerebral artery occlusion in mice
- 陆军军医大学学报 2009年31卷第10期页码：910-913
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  中图分类号： R394.1;R741.02;R743.31
- 纸质出版：2009
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田洪,段炜,桂莉,郑健.腺苷A2A受体在小鼠大脑中动脉局灶缺血损伤中的作用及机制[J].陆军军医大学学报(原第三军医大学学报),2009,31(10):910-913.

　TIAN Hong,DUAN Wei,GUI Li,ZHENG Jian.Role of adenosine A2A receptor in ischemic brain injury induced by transient middle cerebral artery occlusion in mice[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(10):910-913.
田洪,段炜,桂莉,郑健.腺苷A2A受体在小鼠大脑中动脉局灶缺血损伤中的作用及机制[J].陆军军医大学学报(原第三军医大学学报),2009,31(10):910-913. DOI：

　TIAN Hong,DUAN Wei,GUI Li,ZHENG Jian.Role of adenosine A2A receptor in ischemic brain injury induced by transient middle cerebral artery occlusion in mice[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(10):910-913. DOI：

摘要

目的探讨阻断腺苷A

受体对神经元缺血性损伤的影响及其可能机制。方法将腺苷A

受体基因敲除（A

RKO）小鼠24只及同窝野生型（A

RWT）小鼠24只分为A

RKO组和A

RWT组，每组下设脑缺血2 h（各6只）、脑缺血后再灌注22 h（各6只）和脑缺血后再灌注46 h（各12只）3个时相点，另外9只A

RWT小鼠设为假手术组。线栓法制作大脑中动脉阻塞（middle cerebral artery occlusion

MCAO）模型，脑组织切片甲酚紫染色结合图象分析技术测定脑梗死体积，干湿质量法测定脑组织含水量，免疫组化染色检测钙结合蛋白（Calbindin D-28k，CB）和水通道蛋白4（aquaporin，AQP4）。结果在脑缺血2 h、脑缺血后再灌注22 h和脑缺血后再灌注46 h，测定A

RKO 组的脑梗死体积均小于A

RWT组的梗死体积；与A

RWT组比较，A

RKO组脑内CB表达较多，AQP4表达较少

脑含水量较低。结论阻断腺苷A

受体对脑缺血急性期和再灌注期损伤均有保护作用，并减轻缺血引起的脑水肿，可能与抑制细胞内钙超载和AQP4的表达有关。

Abstract

Objective To investigate the effect of adenosine A

receptor deficiency on the ischemic neuronal injury and its potential mechanism. Methods Transient (2 h) cerebral ischemia was induced by the middle cerebral artery occlusion (MCAO) in mice. Adenosine A

receptor knockout (A

RKO) mice and their wild-type littermates (A

RWT) were divided into 2-hour cerebral ischemia group

cerebral ischemia with 22-hour reperfusion group and cerebral ischemia with 46-hour reperfusion group. Cerebral infarction volume was measured by image analysis of brain sections stained with cresyl violet (CV). Brain water content was evaluated with the dry-wet weighing method. The expression of calbindin D-28k (CB) and aquaporin-4 (AQP4) in ischemic brain was determined with immunohistochemical methods. Results The cerebral infarction volumes in 2-hour cerebral ischemia group

cerebral ischemia with 22-hour reperfusion group and cerebral ischemia with 46-hour reperfusion group of A

RKO mice were lesser than those in the corresponding groups of A

RWT mice. Compared with A

2A

RWT mice

RKO mice had more CB

lesser AQP4 expressions and lesser brain water contents. Conclusion Adenosine A

receptor deficiency exerts the protection against ischemic brain injury both in the acute phase and reperfusion phase

and attenuates brain edema caused by cerebral ischemia

which may be due to the inhibition of intracellular calcium overload and AQP4 expression.

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腺苷A2A受体在小鼠大脑中动脉局灶缺血损伤中的作用及机制

Role of adenosine A2A receptor in ischemic brain injury induced by transient middle cerebral artery occlusion in mice

DOI：

摘要

Abstract

关键词

Keywords

references

腺苷A_2A受体在小鼠大脑中动脉局灶缺血损伤中的作用及机制

Role of adenosine A_2A receptor in ischemic brain injury induced by transient middle cerebral artery occlusion in mice