Objective   To study the effect of recombinant adenovirus vector-mediated Livin siRNA on the proliferation

apoptosis and drug resistance in K562 cells.    Methods   The targeting Livin siRNA was subcloned into the shuttle plasmid pSES-HUS

and then recombinant it with adenovirus skeleton plasmid pAdeasy by homologous recombination. We transfected the product into HEK293 cells via liposome and generated recombinant adenovirus Ad5-Livin siRNA. K562 cells were infected with Ad5-Livin siRNA. The gene silencing efficiency of recombinant adenovirus Ad5-Livin siRNA was monitored by real-time PCR and Western blot analysis in K562 cells. The relationship between Livin siRNA and K562 cells was detected by MTT assays and flow cytometry.    Results   The recombinant adenovius vector of Ad5-Livin siRNA was successfully constructed and infected into K562 cells. Recombinant adenovirus vector silenced Livin gene at the level of mRNA and protein

inhibited proliferation and enhanced apoptosis and sensitivity to Adriamycin

Vincristine and Etoposide in K562 cells.     Conclusion   The recombinant adenovirus Ad5-Livin siRNA effectively silences the expression of Livin gene and enhances the apoptosis and sensitivity to anticancer drugs in K562 cells. Livin is a new molecular target of leukemia’s therapy.