Objective    To investigate the effect of recombinant adenovirus containing CD82/KAI1 on the expressions of integrin α5

integrin β3

MMP-9

E-cadherin and β-catenin in uterine stromal cells.     Methods    Uterine stromal cells were isolated from pregnant mice during implantation window and cultured primarily. The recombinant adenovirus containing mouse CD82/KAI1 gene was constructed. Protein expres

sion of integrin α5

integrin β3

MMP-9

E-cadherin and β-catenin were detected by immunocytochemical methods in the cultured cells with or without CD82/KAI1 adenovirus transfection.     Results    When the cells which were cultured for 48 h were transfected with the recombinant adenovirus vector at a titer of 6.5×10

12

pfu/ml

CD82/KAI1 were obviously upregulated in the primary uterine stromal cells. In the uterine stromal cells from pregnant mice on the 4 d after gestation

integrin α5

E-cadherin

β-catenin and MMP-9 were detected

while

integrin β3 were not. But in the cells of nonpregant rats

no expression of these 5 proteins was found. Compared with the cells transfected with blank adenovirus

the recombinant adenovirus encoding CD82/KAI1 upregulated the expressions of β-catenin and MMP-9 (P0.05)

and down-regulated the expressions of integrin α5 (P0.05)

but there was no obvious change in E-cadherin expression (P0.05) and no integrin β3 was detected in the stromal cells of pregnant mice on day 4 of gestation.     Conclusion    The recombinant adenovirus containing mouse CD82/KAI1 gene is successfully constructed. CD82/KAI1 might have certain effect on the expressions of integrin α5

MMP-9

E-cadherin and β-catenin in mouse uterine stromal cells before embryo implantation.