Objective   To clone the cDNA fragment of thioester-containing protein 1 (TEP1) of Anopheles dirus and analyze the sequence with bioinformatic methods.    Methods   The target cDNA fragment was amplified by RT-PCR with degenerated primers designated based on the conserved amino acid sequences of TEP1 of Anopheles gambiae and other insects. And total RNA from haemocytes of Anopheles dirus was used as the template of RT-PCR. The PCR product was purified and T/A cloned

followed by sequencing the positive clone. Then the sequence was analyzed with DNASIS software and the amino acid sequence was deduced. The obtained amino acid sequence was aligned with TEP1 amino acid sequences of the other insects with BLAST and DNASIS.     Results   A band of approximately 770 bp was amplified by RT-PCR. A nucleotide acid sequence with 774 bp in length was obtained by T/A cloning and sequencing. The deduced amino acid sequence was 258 aa in length

and had a sequence homology of 72%

72% and 59%  respectively when compared with the TEP1 sequences of Anopheles gambiae

Anopheles arabiensis and Anopheles stephensi.    Conclusion   The cDNA fragment of TEP1 is successfully cloned from the haemocytes of Anopheles dirus

which provides a foundation for further study on function of the molecular.