Objective    To explore the effects of survivin-targeted small interference RNA (siRNA) on the sensitivity of K562 cell line to adriamycin (ADM).     Methods    K562 cells were divided into three groups: RNA interference (RNAi) group

lipofectamine group and control group. Cell transfection was induced by lipofectamine2000. The expressions of survivin in K562 cells were assayed 48 h after transfection by RT-PCR and immunohistochemical methods. The protein levels of P-glycoprotein (P-gp)

TOPO-2

GST-π were detected using immunohistochemical methods. The sensitivity of K562 cells to ADM was determined by MTT and flow cytometry.     Results    After transfection with recombinant plasmid pshRNA-survivin

the mRNA and protein expressions of survivin in K562 cells were decreased significantly (P0.05). The sensitivity of K562 cells to ADM was increased in RNAi group as compared with lipofectamine group and control group (P0.05). The protein level of GST-π expression was significantly lower in RNAi group than those in the other two control groups (P0.05).     Conclusion    Survivin-targeted RNA interference can increase the sensitivity of K562 cells to ADM

probably by decreasing the expression of GST-π.