Objective    To establish a model of classical eyeblink conditioned reflex (EBCR) in conscious and unfixed guinea pigs and to analyze the related acquisition rule.     Methods    With the aid of metal electrode embedding technique and simple follow-up air puff stimulus facility

conscious and unfixed guinea pigs were trained to performed EBCR with tone as conditioned stimulus (CS) and air puff as  unconditioned stimulus (US). The eyeblink behavior was evaluated through electromyography (EMG) of musculi oculi.     Results    The eyeblink EMG signal of musculi oculi were successfully collected

and the classical eyeblink conditioning was obtained. The acquisition rate in the 4th

7th

and 10th day were 31%

61.3% and 82.7% respectively

and the maintain rate in the 15th and 20th day were 81.3% and 82.3%.     Conclusion    Conscious and unfixed guinea pigs can be trained to obtain EBCR with the techniques of musculi oculi EMG signal recording and follow-up air puff stimulating method. It is an easy

convenient

feasible

and reliable method with high achievement ratio. It could be applied to study conditioned eyeblink behavior of animals with big body type

or animals uneasy to be fixed.