Objective    To study the dynamic changes of transendothelial electrical resistance (TEER) on our in vitro model of brain blood barrier (BBB) made from primary culture of BALB/c mouse brain microvascular endothelial cells (BMVEC)

and to explore the relation between TEER and BBB permeability

and search for the best culture condition.     Methods    BMVEC were isolated from BALB/c mouse and cultured on a transwell insert with special micro-pore. The cells were identified with immunohistochemical methods and electron microscope. TEER over BMVEC was measured after BBB model estab

lishment for determining the

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H-Glucose permeability of BBB in vitro.     Results    BMVEC cultured in the transwell insert exhibited typical “flagstone” appearance and in a tight monolayer structure under electron microscope. Immunohistochemical detection of ZO-1 protein

a marker antigen of tight junction

showed smooth

continuous and tight junctions between confluent BMVEC. TEER over BMVEC monolayer increased to (346±10) Ω/cm

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when the permeability for

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H-Glucose was decreased to the minimum.     Conclusion    BBB model in vitro made from primary culture of BMVEC in transwell has the basic characteristics of BBB in morphology

electrical resistance and permeability.