Objective    To investigate the change of monocyte autophagy in patients after trauma.     Methods     ①According to Injury Severity Score (ISS) or Abbreviated Injury Scale (AIS)

we divided our 51 subjected trauma patients into 3 groups

the minor injury group (n=21)

the moderate trauma group (n=19) and the   severe trauma   group (n=11). Another 14 healthy volunteers served as control. We   collected the peripheral blood in 24 h

3

5 and 10 d after trauma

then harvested the monocytes by density gradient centrifugation with adherence separation. Then we observed the autophagy phenomenon by using laser confocal microscopy and transmission electron microscopy; ② After the isolated monocytes were dyed with MDC and lysed

they were detected and quantified for fluorescent intensity by fluorospectrophotometer (excitation wavelength 380 nm and emission wavelength 525 nm); ③Blood sugar level of all patients were measured in 24 h

3 and 5 d respectively after trauma

and the correlation between autophagy and blood sugar was analyzed.     Results    Compared with control group

more autophagy in monocytes was observed and quantified in trauma patients than in health volunteers (P0.01).     Conclusion    Autophagy in monocytes is associated with the severity of trauma

and has a positive correlation with blood sugar.