Objective    To investigate whether BC047440 gene can induce HepG2 proliferation through enhancing NF-κB activity.     Methods    Small interfering RNAs for BC047440 gene were designed using Ambion software and constructed into pGenesil-1. The pGenesil-1-HK-shRNA and pGenesil-1-BC047440-shRNA vector were all transfected into HepG2 cells using Lipofectamine

TM

2000. The cell colony formation rate was measured by plate colony formation assay. The protien of NF-κB signal pathway was determined by Western blotting. Nuclear translocation and NF-κB activity were eval

uated by electrophoretic mobility shift assay (EMSA) and luciferase reporter assay

respectively.     Results    When the cell colony formation rates were statistically decreased in siRNA treated groups

Western blot analysis revealed decreased expression of p50 in BC047440 gene-silencing HepG2 compared with that of wild HepG2. The levels of nuclear translocation of NF-κB in BC047440 gene-silencing HepG2 were also significantly suppressed compared with that of wild HepG2

and the ratio of luciferase/Renilla Luciferase in BC047440 gene-silencing HepG2 was only 25% of wild HepG2.     Conclusion    BC047440 can induce HepG2 proliferation through NF-κB signal pathway

and BC047440 might be a target of inhibiting the development of hepatocellular carcinoma.