Objective    To study the effect of Cad-Ⅱ gene (OB-Cadherin) transfection on the adhesion and proliferation of human marrow mesenchymal stem cells (hMSCs) cultured on xenogeneic demineralized bone matrix.     Methods    The Cad-Ⅱ gene was transfected into hMSCs

and those without transfection served as control group. Then they were seeded onto the xenogeneic demineralized bone matrix respectively and cocultured in vitro. The densities of seeded cells and adhesion rate was measured

the adhesion with bone matrix complex was also observed by contrast microscopy and electron scanning microscopy. And the cell proliferation was measured with MTT method.     Results    The 2 groups of hMSCs adhered to the surface and inner walls of bone matrix

and spread extensively

with no significant difference in cell proliferation between them. But in the transfection group there were more adhesion cells on the scaffolds

and the adhesion rate (84.5%) was significantly higher than that in control group (71.7%

P0.05).     Conclusion    The Cad-Ⅱgene transfection may enhance the seeded cells adhesion to scaffolds

but has no obvious effect on cell proliferation.