Objective    To study the effects of HYAL1 gene overexpression on invasive

angiogenic and proliferative ability of breast cancer cell lines MCF-7 and ZR-75-30.     Methods    Double-chamber co-culture technique was applied to construct the invasive model and angiogenic model in vitro

which was used to detect the invasive and angiogenic potential of breast cancer cell; MTT and flow cytometry were used to detect the proliferation of breast cancer cells.     Results    Breast cancer cells overexpressing HYAL1 gene showed stronger invasive potential and angiogenic potential than control cells

but had no significant difference on proliferative potential.      Conclusion    Overexpression of HYAL1 gene can promote the invasion and angiogenesis of breast cancer cells in vitro

but not affect the proliferation.