Objective    To investigate the expressions and significance of PAX-5

OCT-2 and BOB.1 in Hodgkin lymphoma (HL).     Methods    Immunohistochemical method was used to detect the expressions of CD30

CD15

PAX-5

OCT-2

BOB.1

CD20 and CD3 in 44 samples of formalin-fixed

paraffin-embedded tissues of HL. The histological classification was carried out according to the WHO classification for tumors of hematopoietic and lymphoid tissues (2001).     Results    In all 44 cases

3 (6.8%) were nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) and 41 (93.2%) were classical Hodgkin lymphoma (CHL). In Hodgkin and Reed Sternberg cells

CD30 were expressed in 36 samples (81.8%)

CD15 in 27 (61.4%)

CD20 in 14 (31.8%)

PAX-5 in 43 (97.7%)

OCT-2 in 14 (31.8%)

and BOB.1 in 7 (15.9%). All samples of NLPHL expressed OCT-2 and BOB.1

while in the samples of CHL

30 samples (73.2%) expressed both OCT-2 and BOB.1

7 (17.1%) expressed only OCT-2

1 (2.4%) expressed only BOB.1 and all were negative for CD30.     Conclusion    Hodgkin lymphoma is a rare neoplasm with uncertain cause and difficult to diagnose. The detection of PAX-5

OCT-2

BOB.1 in combination with CD30

CD15 will conduce to the diagnosis and differential diagnosis of Hodgkin lymphoma.