神经酰胺对人内皮细胞凋亡及bax、bcl-2表达的影响

沈珠甫; 杨丽霞; 郭瑞威; 杨栋; 齐峰; 石燕昆

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神经酰胺对人内皮细胞凋亡及bax、bcl-2表达的影响

论著 | 更新时间：2026-01-09

- 神经酰胺对人内皮细胞凋亡及bax、bcl-2表达的影响
- Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax
- 陆军军医大学学报 2008年30卷第10期页码：957-959
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  中图分类号： R322.123;R329.2;R965
- 纸质出版：2008
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沈珠甫,杨丽霞,郭瑞威,杨栋,齐峰,石燕昆.神经酰胺对人内皮细胞凋亡及bax、bcl-2表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):957-959.

　SHEN Zhu-fu,YANG Li-xia,GUO Rui-wei,YANG Dong,QI Feng,SHI Yan-kun.Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):957-959.
沈珠甫,杨丽霞,郭瑞威,杨栋,齐峰,石燕昆.神经酰胺对人内皮细胞凋亡及bax、bcl-2表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):957-959. DOI：

　SHEN Zhu-fu,YANG Li-xia,GUO Rui-wei,YANG Dong,QI Feng,SHI Yan-kun.Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):957-959. DOI：

摘要

目的观察外源性神经酰胺（C

-ceramide）对内皮细胞凋亡及相关基因bax和bcl-2表达变化的影响。方法原代培养人脐静脉血管内皮细胞，加药组加入终浓度分别为5、12.5、25、50 μmol/L的C

-ceramide，对照组加入体积分数为0.1%的无水乙醇。采用TUNEL法检测细胞凋亡

用RT-PCR、Western blot技术对bax mRNA、bcl-2 mRNA及蛋白表达进行分析。结果加药组内皮细胞的凋亡阳性率显著高于对照组(P0.05)，且具有时间和剂量依赖性；加药组bax mRNA及蛋白的表达较对照组显著升高（P0.05），bcl-2 mRNA及蛋白的表达较对照组则显著降低（P0.05）。结论外源性神经酰胺通过上调bax及下调bcl-2表达水平，改变bax/bcl-2值，从而诱导内皮细胞凋亡。

Abstract

Objective To observe the effect of ceramide on apoptosis of endothelial cells (EC) and determine the relationship between the ceramide-induced growth suppression and the expressions of bax and bcl-2. Methods Human umbilical endothelial cells were cultured in vitro and treated with 5

12.5

50 μmol/L C

-ceramide

the cells treated with absolute alcohol as control. TUNEL was employed to determine the apoptosis of the cultured EC. Bax and bcl-2 mRNA and protein levels of EC were detected by RT-PCR and Western blot. Results The number of apoptotic cells was significantly higher in ceramide-treated endothelial cells than that in the control group (P0

.05). Ceramide-induced EC apopotsis was in time- and dose-dependent manner. Compared with the control cells

the expressions of bax mRNA and protein in EC treated with ceramide significantly increased (P0.05)

while the expressions of bcl-2 mRNA and protein significantly depressed (P0.05). Conclusion Ceramide may induce apoptosis of endothelial cells by down-regulating bcl-2 and up-regulating bax.

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