Objective    To study the biological effects of four isoflavone derivatives (F8

F11

ZF3 and ZF7) on endometrial epithelial cells.     Methods    The endometrial epithelial cells were cultured through collagenase enzymatic digestion and twice grit filtration. The biological effects on endometrial epithelial cells of four isoflavone derivatives were compared through MTT.     Results    The primary endometrial epithelial cells were successfully disassociated

cultured and passaged down stably.  Cell proliferation was significantly increased by F8 (25  mol/L) (P0.05)

but significantly inhibited by F11 (50 and 100  mol/L) (P0.05) 24 h after four isoflavone derivatives acting on endometrial epithelial cells. Cell proliferation was significantly inhibited by F11

ZF3

ZF7 (50  mol/L) (P0.05)and all the 4 isoflavone derivatives (100  mol/L)  48 h later.  Cell proliferation

not inhibited by F8

ZF3 and ZF7 (25  mol/L)

can be inhibited by four isoflavone derivatives of other dosage (P0.05) 72 h later. Their biological effects are time- and dose- dependent.     Conclusion    All the 4 isoflavone derivatives can inhibit the cell proliferation in vitro

among which F11 has the strongest effect.