Objective    To observe and detect catheter induced blood vessel injury by establishing the mouse model.     Methods    Catheter guidewire was inserted and turned around in left common carotid artery to induce vascular injury in mice. After 1

3

4 weeks

the injured vessel was sectioned

the pathological changes of  vessel was checked by HE stain

and the expression of αVβ3 and Ki67 positive cells in the sections were detected by immunohistochemical stain.

111

In-RP478

a specific small ligand for αVβ3 was injected into injured mice to separate the injured vessel from the non-injured vessel 2.5 h later

and  their autoradiography was measured.     Results    Neointima in the injured left common carotid w

as shown up and the media thickened significantly after injury. Expression of αVβ3

mainly in neointima

were increased after injury and a lot of Ki67 positive cells were found in neointima and media of the injured vessel. Intensity of injured autography was much higher than that of control.  The intensity ratio of injured left common carotid artery to non-injured was in positive relationship with the degree of injury.     Conclusion    Catheter could induce vascular injury

and RP478 could be a good tracer for the injured vessel.