Objective    To study the effect of soluble TRAIL protein (sTRAIL) on the gastric cancer cell line BGC-823.     Methods    EGFP as report gene and sTRAIL as the target gene

a double expression plasmid of pBud-EGFP-TRAIL was constructed. After identified by restriction digestion and sequencing

pBud-EGFP-TRAIL was transfected into gastric cancer cell line BGC-823 by Lipofectamine

TM

2000. The expression of EGFP was observed by fluorescent microscope. The mRNA and protein expressions of sTRAIL were detected by RT-PCR and Western blot. The proliferation of BGC-823 cells was examined by MTT method

and the apoptosis of BGC-823 cells wa

s examined by TUNEL method and flow cytometry.     Results    Sequencing result showed that the recombinant plasmid of pBud-EGFP-TRAIL had been constructed successfully. After transfected with pBud-EGFP-TRAIL

BGC-823 cells expressed sTRAIL protein and their apoptosis was induced. The inhibition rate of proliferation of BGC-823 cells transfected with pBud-EGFP-TRAIL was higher than that of the control by MTT

and the apoptosis rate was also higher by flow cytometry. Karyopycnosis

nuclear chromosome condensation and nuclear segmentation were observed by TUNEL.     Conclusion    It is proved that sTRAIL can cause apoptosis of gastric cancer cell line BGC-823.