Objective    To investigate the effects of nordihydroguaiaretic acid (NDGA) on the endometriosis established in Bama miniature pigs.     Methods    Six Bama pigs that had been successfully established into animal model of endometriosis subcutaneously received 3% NDGA at dose of 20 mg/kg (n=4) or PBS solution (n=2) for 20 d. The grafts in abdominal cavity and subcutaneous tissues were observed and the serum concentrations of hormone

  were measured by chemiluminoimmunoassay before and after the period of NDGA or PBS injection. The expressions of FⅧAg and VEGF in the endometriotic tissues were observed.     Results    The endometriotic masses were reduced to some

degree after the time period of NDGA injection

even partially disappeared

while those treated with PBS showed no obvious changes. Histoimmunochemical analysis showed NDGA decreased the number of endometrial glands

the proliferation rate of the glandular epithelial cells

the endometrial stroma cells and the vessels

and inhibited the microvascular density and VEGF in the endometriotic tissues (P0.05). The serum concentration of E

2

 decreased significantly after the whole course of NDGA injection (P0.05).     Conclusion    NDGA can reduce the endometriotic mass or even make partial endometriotic mass disappear.