Objective    To investigate the function of mesothelin by analyzing the location

the expressions and the adhesive ability of mesothelin and CA125 in human epithelial ovarian cancer.     Methods    Mesothelin and CA125 protein expressions and locations in human epithelial ovarian cancer tissues and cell lines were determined by indirect immunofluorescence double maker and Western blot.  Cell adhesive ability was detected by EDTA-induced cell detachment.     Results    Mesothelin and CA125 were specifically located in cytomembrane of human epithelial ovarian cancer cells. Expressions of  mesothelin in the  SKOV-3 and 3AO cells were weaker than those in the OVCAR-3.  SKOV-3 and 3AO cells adhered less effectively to plastic substance and matrigel than OVCAR-3 (P0.01).     Conclusion    Different expressions and co-location of mesothelin and CA125 were found in in the 3 kinds of human epithelial ovarian cancer tissues and cell lines. The expression level of mesothelin may be related to cell adhesive ability.