Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究

刘川; 吴小候; 张唯力; 唐伟; 尹志康; 何云锋; 杜虎

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Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究

论著 | 更新时间：2026-01-09

- Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究
- Antisense oligonucleotide targeting livin induces apoptosis of human renal carcinoma cell 786-O in vitro and its mechanism
- 陆军军医大学学报 2008年30卷第17期页码：1622-1625
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  中图分类号： R394.2;R73-362;R737.11
- 纸质出版：2008
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刘川,吴小候,张唯力,唐伟,尹志康,何云锋,杜虎.Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1622-1625.

　LIU Chuan,WU Xiao-hou,ZHANG Wei-li,TANG Wei,YIN Zhi-kang,HE Yun-feng,DU Hu.Antisense oligonucleotide targeting livin induces apoptosis of human renal carcinoma cell 786-O in vitro and its mechanism[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(17):1622-1625.
刘川,吴小候,张唯力,唐伟,尹志康,何云锋,杜虎.Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1622-1625. DOI：

　LIU Chuan,WU Xiao-hou,ZHANG Wei-li,TANG Wei,YIN Zhi-kang,HE Yun-feng,DU Hu.Antisense oligonucleotide targeting livin induces apoptosis of human renal carcinoma cell 786-O in vitro and its mechanism[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(17):1622-1625. DOI：

摘要

目的探讨Livin反义寡核苷酸(ASODN)对人肾癌细胞株786-O Livin mRNA及蛋白表达的抑制作用以及对细胞凋亡的影响。方法设计合成全硫代磷酸化修饰的Livin ASODN，脂质体辅助转染至786-O细胞内。采用RT-PCR检测转染后Livin mRNA的变化，细胞免疫化学、激光扫描共聚焦显微镜观察Livin蛋白在细胞内的分布、转染后表达的变化及细胞形态的变化，流式细胞仪检测细胞凋亡率的变化，激酶法测定Caspase3活性的变化。结果转染Livin ASODN后，RT-PCR显示786-O细胞表达Livin mRNA减少(P0.01)，激光扫描共聚焦显微镜下见细胞表达Livin蛋白减少、细胞形态发生变化，流式细胞仪检测细胞凋亡率显著增加(P0.01)，Caspase3活性明显增加(P0.01)。结论 Livin ASODN能明显下调786-O细胞Livin基因的表达，诱导细胞凋亡。抑制Caspase3活性是Livin抑制细胞凋亡的途径之一。

Abstract

Objective To investigate the effect of antisense oligonucleotides (ASODN) targeting livin on the inhibition of livin mRNA and protein expression and the apoptosis of human renal carcinoma cell line 786-O cells. Methods Specific phosphorothioate antisense oligodeoxynucleotides targeting livin were synthesized and then transfected into 786-O cells. The expressions of livin mRNA were detected by RT-PCR. Expression and location of livin protein were observed by confocal laser scanning microscope (CLSM). Apoptosis rate of 786-O cells was investigated by flow cytometer. The activity of Caspase-3 was detected by colorimetric assay. Results After the transfection of ASODN

the expression of livin mRNA was decreased (P0.01)

the fluorescence signal of livin protein observed by CLSM was significantly decreased

the apoptosis rates detected by flow cytometry were significantly increased (P0.01)

and the caspase-3 activity was enhanced (P0.01). Conclusion Livin ASODN could remarkably down-regulate the expression of livin gene in 786-O cells line and induce tumor cell apoptosis. These events are linked to the activation of caspase-3.

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