Objective    To screen proteins related with liver regeneration by studying the proteomic difference between the hepatocytes 6 h and 24 h after the 70% hepatotectmy and the normal ones.     Methods    Nine male SD rats (weight 200 g) were selected and randomly divided into 3 groups: the control group

the 6 h group and the 24 h group

each one with 3 rats. The rats 70%  hepatectomy model was built.  The hepatocytes in the control group were immediately isolated from the rat liver and those in the 6 h group and 24 h group were isolated after 6 h and 24 h respectively. We extracted the total proteins with cell lysis and anselyzed the proteomic differences between the 3 groups.     Results    We found 47 different proteins and identified 42 of them.     Conclusion    The results indicated that the proteins produced in the 24 h group are to a large extent identical with those in the 6 h group and the control group

however

the proteins produced in the 6 h group are seldom the same with those in the control group. This is because the hepatocytes in the 6 h group was in the start state of liver regeneration while the hepatocytes in the 24 h not only produced proteins related to the liver regeneration but also the proteins maintaining the normal physiological function of the hepatocytes.