外源性Wnt5a激活K562细胞非经典Wnt5a/Ca<sup>2+</sup>信号途径研究

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外源性Wnt5a激活K562细胞非经典Wnt5a/Ca²⁺信号途径研究

论著 | 更新时间：2026-01-09

- 外源性Wnt5a激活K562细胞非经典Wnt5a/Ca²⁺信号途径研究
- Noncanonical Wnt5a/Ca²⁺ pathway activated by exogenous Wnt5a in K562 cells
- 陆军军医大学学报 2008年30卷第10期页码：924-927
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  中图分类号： R329.28;R730.33;R733.7
- 纸质出版：2008
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李招权,司维柯,邹全明,袁媛,潘静,赵宸.外源性Wnt5a激活K562细胞非经典Wnt5a/Ca2+信号途径研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):924-927.

　LI Zhao-quan,SI Wei-ke,ZOU Quan-ming,YUAN Yuan,PAN Jing,ZHAO Chen.Noncanonical Wnt5a/Ca2+ pathway activated by exogenous Wnt5a in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):924-927.
李招权,司维柯,邹全明,袁媛,潘静,赵宸.外源性Wnt5a激活K562细胞非经典Wnt5a/Ca2+信号途径研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):924-927. DOI：

　LI Zhao-quan,SI Wei-ke,ZOU Quan-ming,YUAN Yuan,PAN Jing,ZHAO Chen.Noncanonical Wnt5a/Ca2+ pathway activated by exogenous Wnt5a in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):924-927. DOI：

目的观察外源性Wat5a对K562细胞非经典Wnt5a/Ca

途径的影响，进一步研究外源性Wnt5a诱导K562细胞的分化机制。方法激光共聚焦显微镜观察Ca

内流的变化，以RT-PCR及免疫组化检测β-catenin的表达变化，以Western blot检测cyclin D1的表达变化。结果外源性Wnt5a能明显促进K562细胞Ca

内流，显著下调cyclin D1表达，且能下调K562细胞β-catenin表达，但β-catenin表达下调无统计学意义。结论外源性Wnt5a能激活K562细胞非经典Wnt5a/Ca

途径，外源性Wnt5a诱导K562细胞分化机制可能与此相关。

Objective To study the mechanism of K562 cell differentiation induced by exogenous Wnt5a in aspect of noncanonical Wnt5a/Ca

signaling pathway. Methods After K562 cells were cultured in medium supplemented with Wnt5a- or GFP-riched supernatant for 1-5 d

inflow changes were observed by laser confocal microscopy. The expression changes of β-catenin and cyclin D1 were detected by RT-PCR

immunocytochemical staining and Western blotting. Results Exogenous Wnt5a obviously promoted the Ca

inflow of K562 cells and down-regulated the expression of cyclin D1. Furthermore

the expression of β-catenin was lower

but had no statistically significance. Conclu

sion Exogenous Wnt5a can activate noncanonical Wnt5a/Ca

signaling pathway in K562 cells

which may relate to the K562 cell differentiation.

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