Objective   To verify the differential expression of the protein－guanine nucleotide-binding protein subunit beta 2-like 1 (GBLP) that has been identified by comparative proteomics methods.    Methods   Human umbilical vein endothelial cells (HUVEC) were treated with R/S-propranolol (R/S-PRO) respectively for 3 h

then the differentially expressed GBLP protein was isolated and identified by comparative proteomics methods. Western blot analysis and the confocal laser scanning microscopy (LSCM) were used to further verify the expression abundance and display location of GBLP in two enantiomers treated HUVEC.    Results   Both results of Western blot and LSCM exhibited that the expression abundance of GBLP in S-PRO treated HUVEC was lower than that in R-PRO treated cells. GBLP located in cytoplasm and plasma membrane in HUVEC

homogeneously distributed in cytoplasm and aggregatively distributed in plasma membrane.    Conclusion   The differential expression of GBLP proven by Western blot and LSCM is consistent with that identified by proteomics methods.