Objective  To investigate the expression of BMP-7 gene modified bone marrow mesenchymal stem cells（BMSCs） after mice were exposed to

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Co gamma ray.    Methods  The recombinant adeno-associated virus vectors encoding BMP-7 (pAAV-BMP-7) were constructed with the technique of molecular cloning. The recombinant expression plasmid were co-transfected into HEK-293 cells with pHelper and pAAV-RC by calcium-phosphate precipitation method. Recombinant AAV viral particles from infected HEK-293 cells were used to infect BMSCs. The infected MMSCs were injected into mice through caudal vein after the BALB/c mice were exposed to 8.0 Gy

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Co γ ray. BMP-7 expressions were observed by immunocytochemical staining in the kidneys of control

mice only exposed to γ ray and mice transfected with BMSCs or BMP-7 gene modified BMSCs.   Results  The recombinant AAV vectors of BMP-7 gene were constructed and expressed in BMSCs successfully. The infected BMSCs could lodge in the kidneys.   Conclusion  These genetically modified BMSCs might be used for further research on gene therapy of renal diseases.