血管紧张素Ⅱ诱导成人脂肪间充质干细胞分化为心肌样细胞的实验研究

张卫泽; 樊艳; 陈永清; 马凌; 哈小琴; 张耀辉

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血管紧张素Ⅱ诱导成人脂肪间充质干细胞分化为心肌样细胞的实验研究

论著 | 更新时间：2026-01-09

- 血管紧张素Ⅱ诱导成人脂肪间充质干细胞分化为心肌样细胞的实验研究
- Angiotensin Ⅱ induces adult adipose-derived mesenchymal stem cells to differentiate into cardiocyte-like cells
- 陆军军医大学学报 2008年30卷第19期页码：1795-1798
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  中图分类号： R322.11;R392.1
- 纸质出版：2008
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张卫泽,樊艳,陈永清,马凌,哈小琴,张耀辉.血管紧张素Ⅱ诱导成人脂肪间充质干细胞分化为心肌样细胞的实验研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(19):1795-1798.

　ZHANG Wei-ze,FAN Yan,CHEN Yong-qing,MA Ling,HA Xiao-qin,ZHANG Yao-hui.Angiotensin Ⅱ induces adult adipose-derived mesenchymal stem cells to differentiate into cardiocyte-like cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(19):1795-1798.
张卫泽,樊艳,陈永清,马凌,哈小琴,张耀辉.血管紧张素Ⅱ诱导成人脂肪间充质干细胞分化为心肌样细胞的实验研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(19):1795-1798. DOI：

　ZHANG Wei-ze,FAN Yan,CHEN Yong-qing,MA Ling,HA Xiao-qin,ZHANG Yao-hui.Angiotensin Ⅱ induces adult adipose-derived mesenchymal stem cells to differentiate into cardiocyte-like cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(19):1795-1798. DOI：

摘要

目的采用血管紧张素Ⅱ（angiotensin Ⅱ

AngⅡ）作为诱导剂，体外诱导成人脂肪间充质干细胞（adipose-derived mesenchymal stem cells

ADMSCs）向心肌细胞方向分化。方法自成人脂肪组织中分离培养ADMSCs，分别用0.05、0.10和0.20 μmol/L 3种不同浓度的AngⅡ诱导ADMSCs，同时设立对照组，倒置相差显微镜及透射电子显微镜下观察细胞的形态学特征，免疫细胞化学染色方法检测心肌特异性肌钙蛋白-I（cardiac troponin-I

cTn-I），计算转化率。结果诱导后第3周时，0.20 μmol/L组可见cTn-I阳性细胞表达，第4周时0.10 μmol/L组和0.20 μmol/L组均有cTn-I阳性细胞表达，但0.20 μmol/L组的阳性细胞数明显多于前组，且细胞形态变化显著。结论 AngⅡ在体外可以诱导ADMSCs分化为心肌样细胞，其诱导分化的最佳浓度为0.20 μmol/L。

Abstract

Objective To study the effects of angiotensin Ⅱ (AngⅡ)

as inducing agent in vitro

in inducing adult adipose-derived mesenchymal stem cells (ADMSCs) to the direction of myocardial cell. Methods ADMSCs were isolated and cultured from adult adipose tissues. Different concentrations of AngⅡ (0.05

0.10 and 0.20 μmol/L

respectively) were used to induce ADMSCs. Untreated ADMSCs were used as normal control. The cell morphological feature was observed under inverted phase-contrast microscope and transmission electron microscope. Immunocytochemical staining was used to detect the cardiac troponin-I (cTn-I) and calculate the conversion rate. Results After three weeks of induction

cTn-I-positive cells could be detected in 0.20 μmol/L AngⅡ group. Four weeks later

cTn-I-positive cells were detected in 0.10 and 0.20 μmol/L AngⅡ groups

furthermore

the number of cTn-I-positive cells in 0.20 μmol/L AngⅡ group was significantly higher than that of other groups. Conclusion Ang Ⅱ can induce in vitro ADMSCs to differentiate into cardiocyte-like cells

and the best induction concentration is 0.20 μmol/L.

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