Objective    To investigate the multilineage differentiation capacity of mesenchymal stem cells isolated and cultured from swine adipose tissue.     Methods    Adipose tissue-derived stromal cells (ADSCs) were obtained from adipose tissue of swine abdominal wall. The cells presented fibroblast-like in the culture medium. Adipose tissue was minced and digested with collagenase type Ⅰ. The obtained cells were plated and expanded in DMEM/F12 medium. Then the cells were cultivated in osteoblast-inducing culture medium

and osteoblast phenotype was assayed with Gomori and von Kossa staining. The passage cells were cultured in adipogenisis medium and stained with Oil Red O for identification. The cells were observed with inverted microscope each day.     Results    ADSCs grew as adherent cells and presented fibroblast-like in vitro

and could stably proliferate and be passed. Polygon-shaped cells were characterized by large size with a relatively large nucleus and plenty of cytoplasm after osteoblast-inducing. The cells changed from fibroblast-like into ellipse after adipogenisis-inducing cultivation. There were a lot of alkaline phosphatase (ALP) particles by Gomori staining. After induced for 15 and 19 d

positive cells for ALP were (25.0±7.5)％and (49.7±6.9)％

respectively. Von Kossa staining demonstrated the formation of mineralized nods in extracellular matrix. Under the inverted microscope

there existed significant lipid drops around the cell nucleus after adipogenisis-inducing cultivation for 2 d. The adipocytes rate on day 3

7 and 14 was (22.3±12.5)%

(49.6±7.1)% and (89.4±9.3)%

respectively. After 14 d of adipocyte inducing

about (92.5±9.1)% cells displayed accumulation of lipid vacuoles

as detected by Red Oil O.     Conclusion    ADSCs isolated and cultured from swine adipose tissue can be induced to be osteo-inducing and adipogenisis

suggesting that the cells have the multilineage differentiation.