Objective To investigate the influences of temperature

incubation time and

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I-angiostatin (AS) concentration on the internalization of AS receptor in ECV304 cells.  Methods After ECV304 cells were incubated together with

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I-AS at different temperature (4

24 or 37 ℃) for 0.5

1

4

8 or 24h

the amou

nt of membrane receptor that internalized

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I-AS was detected with γ counter. ECV304 cells were incubated with

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I-AS of different concentrations at 37 ℃ for 0.5

1

4

8 or 24 h

then the amount of membrane receptor that internalized

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I-AS was detected.  Results The ratio of internalized

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I-AS was lower at 4 ℃

moderate at 24 ℃

and higher at 37 ℃. With the increasing concentration of

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I-AS

the internalization ratio decreased at 37 ℃. The ratio of AS receptor internalization increased as the incubation time prolonged at any temperature and concentration.  Conclusion Temperature

incubation time and

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I-AS concentration affect the internalization of AS receptor of ECV304 cells. The internalization has a temperature-effective and time-effective relation

and is negative correlated with

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I-AS concentration.