ZHU Hui-li,SHEN Wei,CHEN Zhong-Yong.Apoptosis of hepatic stellate cells induced by sulfasalazine[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(01):70-74.
ZHU Hui-li,SHEN Wei,CHEN Zhong-Yong.Apoptosis of hepatic stellate cells induced by sulfasalazine[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(01):70-74.DOI：
Objective To determine whether sulfasalazine stimulates hepatic stellate cell (HSC-T6) apoptosis and its possible mechanism. Methods CCK-8 assay
acridine orange/ethidium bromide(AO/EB) and Annexin Ⅴ FITC/PI were used to determine cell growth and cell apoptosis. The expression of NF-κB P65
phospho-IKK and phospho-IκB was detected by Western blotting. The nuclear translocation of HSC-T6 P65 was observed with laser confocal microscopy. Results Sulfasalazine displayed a strong growth inhibition and promoting apoptosis effect on HSC-T6 cells in a dose and time-dependent manner. Sulfasalazine
but not 5-aminosalicylic acid or sulfapyridine
inhibited the activation of NF-κB by down-regulating the expressions of PIKK
P-IκB and the nuclear translocation of P65. Conclusion Sulfasalazine can inhibit NF-κB activity and promote apoptosis in HSC-T6 cells
where the Rel/NF-κB/IκB/IKK pathway plays an important role in HSC survival.
京公网安备11010802024621