LUO Tao,HUANG Wen-xiang,WU Li-xian.Establishment of mouse septic peritonitis model with low pathogenic bacteria[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(01):67-69.
LUO Tao,HUANG Wen-xiang,WU Li-xian.Establishment of mouse septic peritonitis model with low pathogenic bacteria[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(01):67-69.DOI：
Objective To apply sterile rat fecal extracts (SRFE) in the establishment of mice septic peritonitis models and evaluate its effect. Methods SRFE and/or 5% yeast were added to dilute the broth of different bacteria
0.5 ml of which was injected into each mouse’s peritoneal cavity. Minimal lethal dose (MLD) was compared when there was SRFE or not. Mortality of mice was compared as the change of concentration of SRFE and different ratio (SRFE: 5% yeast). Results MLD was decreased 78%~90% with the application of SRFE. Mortality of mice was increased with rise of SRFE concentration. The application of SRFE alone was not so effective and 5% yeast was necessary to cooperate with SRFE. Different ratio of SRFE and 5% yeast didn’t affect the mortality of mice (P0.05). Conclusion The application of SRFE can decrease MLD effectively when form mice septic peritonitis models
especially for those bacteria with low pathogenicity.
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