Objective  To observe the effect of keratinocyte growth factor (KGF) on growth and CA125 secretion of cultured endometrial epithelial cells (EECs) from the patients of anovulatory dysfunctional uterine bleeding (ADUB).   Methods  The EECs from 30 cases of ADUB and 22 cases of resected uterus due to other diseases but with normal menstruation were separated and cultured with KGF of different concentration. Their growth curves were drawn by MTT method. Quantity of CA125 in EECs was determined.   Results  EECs from ADUB patients appeared similar to normal EECs and were CK19 positive. KGF increased the proliferation of EECs from ADUB patients significantly (P0.05)

but the proliferation degree was lower than that of normal EECs. KGF increased the CA125 secretion by EECs from ADUB patients significantly (P0.05)

but the degree of CA125 secretion was lower than that of normal EECs. The proliferation of EECs was correlated significantly with CA125 secretion (P0.05).   Conclusion  KGF enhances the growth and CA125 secretion of cultured EECs from ADUB patients

but the degree in EECs from ADUB patients was lower than that of normal EECs. These suggest that EECs from ADUB patients have different growth mechanism with normal EECs.