Objective To explore the molecular mechanism that LRIG1 inhibits signal transduction system of epidermal growth factor receptor (EGFR) by investigating the role of LRIG1 in glioma.   Methods The plasmid pcDNA3.1-LRIG1 was transfected into primary glioma cells by Lipofectamine. Then

the changes of LRJG1 and EGFR in the transfected glioma cells were measured by RT-PCR and Western blot

and the cell proliferation and apoptosis were analyzed by MTT and flow cytometry.    Results The expression levels of LRIG1 mRNA and protein in the glioma cells transfected with pcDNA-LRIG1 were significantly higher than those of control group and pcDNA3.1 transfected glioma cells

while those of EGFR mRNA and protein were significantly lower. The expression of PKCα and Bax was up-regulated

while the expression of bcl-2 was down-regulated. The growth of glioma cells was inhibited and their apoptosis was obviously enhanced.    Conclusion By participating the construction of the negative feedback loop of EGFR

LRIG1 inhibits the occurrence and growth of tumor through several pathways.